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Rare Earth Element Biomining from the Great Salt Lake Brine Using Engineered E. Coli
This data describes rare earth element adsorption onto E. coli cells engineered to express a lanthanide binding tag (LBT). We used a Great Salt Lake synthetic solution as the background matrix with Tb added to 1-10,000 ppb, concentrations much lower than the competing ions presen...
Jiao, Y. et al Lawrence Livermore National Laboratory
Jun 04, 2017
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Great Salt Lake Composition and Rare Earth Speciation Analysis
We have conducted aqueous speciation analyses of the Great Salt Lake (GSL) brine sample (Table 1) and a mock geo sample (Table 2) spiked with 1 ppb Tb and 100 ppb Tb. The GSL speciation (Figure 1) aligns with our basic speciation expectations that strong carbonate complexes would ...
Jiao, Y. et al Lawrence Livermore National Laboratory
Apr 19, 2017
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REE Adsorption Performance with Immobilized Caulobacter Biofilms
This submission includes data collected from experiments on the performance of rare earth adsorption by immobilized bacteria that accompany the FY18 Q2 and Q3 quarter reports. Relevant information from these reports is included in a resource below. The spreadsheet below includes d...
Jiao, Y. and Park, D. Lawrence Livermore National Laboratory
Apr 01, 2018
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Techno-Economic Assessment for Integrating Biosorption into Rare Earth Recovery Process
The current uncertainty in the global supply of rare earth elements (REEs) necessitates the development of novel extraction technologies that utilize a variety of REE source materials. Herein, we examined the techno-economic performance of integrating a biosorption approach into ...
Jiao, Y. et al Lawrence Livermore National Laboratory
Jul 01, 2017
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Techno-Economic Assessment for Integrating Biosorption into Rare Earth Recovery Process
The current uncertainty in the global supply of rare earth elements (REEs) necessitates the development of novel extraction technologies that utilize a variety of REE source materials. Herein, we examined the techno-economic performance of integrating a biosorption approach into a...
Jiao, Y. et al Lawrence Livermore National Laboratory
Oct 09, 2017
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Surface Complexation Modeling of Terbium Biosorption onto E. Coli Bacterial Surfaces with Lanthanide Binding Tags
Lanthanide binding tags (LBTs) have been engineered onto native Escherichia coli (E. coli) bacterial surfaces to enhance extraction and recovery of rare earth elements (REEs). Three strains of E. coli were studied: (1) the native E. coli surface, (2) a mutant E. coli surface with ...
Jiao, Y. Lawrence Livermore National Laboratory
Apr 01, 2018
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Rare Earth Desorption Test with Citrate and Bicarbonate
LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 u...
Jiao, Y. et al Lawrence Livermore National Laboratory
Jun 01, 2017
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Rare Earth Adsorption and Desorption with PEGDA Beads
We synthesized PEGDA polymer hydrogel beads for cell embedding and compared REE biosorption with these beads via a gravity-driven flow through setup. One way to set up a flow through system is by cell encapsulation into polymer beads with a column setup similar to that used in th...
Jiao, Y. et al Lawrence Livermore National Laboratory
Mar 01, 2017
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Recovery of Rare Earth Elements from Geothermal Fluids through Bacterial Cell Surface Adsorption
We summarized the FY17 and part of FY18 results of the analysis of the effect of several parameters (e.g., total dissolved solids, specific competing metals, pH, and temperature) on REE recovery from geothermal brine in a manuscript that was submitted to Environmental Science & Te...
Jiao, Y. et al Lawrence Livermore National Laboratory
Jan 01, 2018
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Design of an Airlift Bioreactor
An important consideration for the process design is cell immobilization-enabled flow-through operation. Large-scale biosorption relies on cells that are immobilized on a supporting substrate and used to 'attract' metal ions. Cell immobilization allows easy separation of the feed...
Jiao, Y. et al Lawrence Livermore National Laboratory
Mar 13, 2017
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Simulating Complex Fracture Systems in Geothermal Reservoirs Using an Explicitly Coupled Hydro-Geomechanical Model
Low permeability geothermal reservoirs can be stimulated by hydraulic fracturing to create Enhanced (or Engineered) Geothermal Systems (EGS) with higher permeability and improved heat transfer to increase heat production. In this paper, we document our effort to develop a numerica...
Carrigan, C. et al Lawrence Livermore National Laboratory
Jan 01, 2011
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Generalized Displacement Correlation Method for Estimating Stress Intensity Factors
This paper presents a generalized form of the displacement correlation method (the GDC method), which can use any linear or quadratic finite element type with homogeneous meshing without local refinement. These two features are critical for modeling dynamic fracture propagation pr...
Fu, P. et al Lawrence Livermore National Laboratory
Jan 01, 2012
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Simulation Tools for Modeling Thermal Spallation Drilling on Multiple Scales
Widespread adoption of geothermal energy will require access to deeply buried resources in granitic basement rocks at high temperatures and pressures. Exploiting these resources necessitates novel methods for drilling, stimulation, and maintenance, under operating conditions that ...
Walsh, S. et al Lawrence Livermore National Laboratory
Jan 01, 2012
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