Rare Earth Desorption Test with Citrate and Bicarbonate
LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.
Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.
Citation Formats
TY - DATA
AB - LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.
Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.
AU - Jiao, Yongqin
A2 - Brewer, Aaron
A3 - Park, Dan
DB - Open Energy Data Initiative (OEDI)
DP - Open EI | National Renewable Energy Laboratory
DO - 10.15121/1452718
KW - geothermal
KW - energy
KW - adsorption
KW - desorption
KW - rare earth
KW - citrate
KW - bicarbonate
KW - REE
KW - resource recovery
KW - centrifuge
KW - brine
KW - lbt
KW - lanthanide binding tag
LA - English
DA - 2017/06/01
PY - 2017
PB - Lawrence Livermore National Laboratory
T1 - Rare Earth Desorption Test with Citrate and Bicarbonate
UR - https://doi.org/10.15121/1452718
ER -
Jiao, Yongqin, et al. Rare Earth Desorption Test with Citrate and Bicarbonate. Lawrence Livermore National Laboratory, 1 June, 2017, GDR. https://doi.org/10.15121/1452718.
Jiao, Y., Brewer, A., & Park, D. (2017). Rare Earth Desorption Test with Citrate and Bicarbonate. [Data set]. GDR. Lawrence Livermore National Laboratory. https://doi.org/10.15121/1452718
Jiao, Yongqin, Aaron Brewer, and Dan Park. Rare Earth Desorption Test with Citrate and Bicarbonate. Lawrence Livermore National Laboratory, June, 1, 2017. Distributed by GDR. https://doi.org/10.15121/1452718
@misc{OEDI_Dataset_7133,
title = {Rare Earth Desorption Test with Citrate and Bicarbonate},
author = {Jiao, Yongqin and Brewer, Aaron and Park, Dan},
abstractNote = {LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05\% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002\% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25\% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.
Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.},
url = {https://gdr.openei.org/submissions/965},
year = {2017},
howpublished = {GDR, Lawrence Livermore National Laboratory, https://doi.org/10.15121/1452718},
note = {Accessed: 2025-05-03},
doi = {10.15121/1452718}
}
https://dx.doi.org/10.15121/1452718
Details
Data from Jun 1, 2017
Last updated Jun 14, 2018
Submitted Sep 5, 2017
Organization
Lawrence Livermore National Laboratory
Contact
Yongqin Jiao
925.422.4482
Authors
Original Source
https://gdr.openei.org/submissions/965Research Areas
Keywords
geothermal, energy, adsorption, desorption, rare earth, citrate, bicarbonate, REE, resource recovery, centrifuge, brine, lbt, lanthanide binding tagDOE Project Details
Project Name Extraction of Rare Earth Metals from Geothermal Fluids using Bioengineered Microbes
Project Lead Holly Thomas
Project Number LLNL FY17 AOP 2.5.1.12
