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Lawrence Livermore National Laboratory×

Rare Earth Desorption Test with Citrate and Bicarbonate

LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 u...
Jiao, Y. et al Lawrence Livermore National Laboratory
Jun 01, 2017
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Design of an Airlift Bioreactor

An important consideration for the process design is cell immobilization-enabled flow-through operation. Large-scale biosorption relies on cells that are immobilized on a supporting substrate and used to 'attract' metal ions. Cell immobilization allows easy separation of the feed...
Jiao, Y. et al Lawrence Livermore National Laboratory
Mar 13, 2017
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Rare Earth Adsorption and Desorption with PEGDA Beads

We synthesized PEGDA polymer hydrogel beads for cell embedding and compared REE biosorption with these beads via a gravity-driven flow through setup. One way to set up a flow through system is by cell encapsulation into polymer beads with a column setup similar to that used in th...
Jiao, Y. et al Lawrence Livermore National Laboratory
Mar 01, 2017
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Recovery of Rare Earth Elements from Geothermal Fluids through Bacterial Cell Surface Adsorption

We summarized the FY17 and part of FY18 results of the analysis of the effect of several parameters (e.g., total dissolved solids, specific competing metals, pH, and temperature) on REE recovery from geothermal brine in a manuscript that was submitted to Environmental Science & Te...
Jiao, Y. et al Lawrence Livermore National Laboratory
Jan 01, 2018
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Surface Complexation Modeling of Terbium Biosorption onto E. Coli Bacterial Surfaces with Lanthanide Binding Tags

Lanthanide binding tags (LBTs) have been engineered onto native Escherichia coli (E. coli) bacterial surfaces to enhance extraction and recovery of rare earth elements (REEs). Three strains of E. coli were studied: (1) the native E. coli surface, (2) a mutant E. coli surface with ...
Jiao, Y. Lawrence Livermore National Laboratory
Apr 01, 2018
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REE Adsorption Performance with Immobilized Caulobacter Biofilms

This submission includes data collected from experiments on the performance of rare earth adsorption by immobilized bacteria that accompany the FY18 Q2 and Q3 quarter reports. Relevant information from these reports is included in a resource below. The spreadsheet below includes d...
Jiao, Y. and Park, D. Lawrence Livermore National Laboratory
Apr 01, 2018
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Utah FORGE 2-2446: Report on Phase Field Modelling of Near-Wellbore Hydraulic Fracture Nucleation and Propagation

This is a report that describes the modelling of fracture nucleation and propagation in the near-wellbore region to understand the relationship between in situ stress and fracture patterns. A novel phase field formulation is described here, which represents fractures as a diffuse ...
Cusini, M. and Fei, F. Lawrence Livermore National Laboratory
Dec 31, 2023
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Conductance Steamflow Relationship

These histograms represent our calibration of conductance of a volcanic geothermal field (with a clay cap) and the observed steam flow rates. Darajat is a vapor geothermal field located in West Java, Indonesia. First production from the field was started in 1994 and additional cap...
Trainor-Guitton, W. Lawrence Livermore National Laboratory
Apr 01, 2015
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