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Lawrence Livermore National Laboratory×

Design of an Airlift Bioreactor

An important consideration for the process design is cell immobilization-enabled flow-through operation. Large-scale biosorption relies on cells that are immobilized on a supporting substrate and used to 'attract' metal ions. Cell immobilization allows easy separation of the feed...
Jiao, Y. et al Lawrence Livermore National Laboratory
Mar 13, 2017
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Rare Earth Adsorption and Desorption with PEGDA Beads

We synthesized PEGDA polymer hydrogel beads for cell embedding and compared REE biosorption with these beads via a gravity-driven flow through setup. One way to set up a flow through system is by cell encapsulation into polymer beads with a column setup similar to that used in th...
Jiao, Y. et al Lawrence Livermore National Laboratory
Mar 01, 2017
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Recovery of Rare Earth Elements from Geothermal Fluids through Bacterial Cell Surface Adsorption

We summarized the FY17 and part of FY18 results of the analysis of the effect of several parameters (e.g., total dissolved solids, specific competing metals, pH, and temperature) on REE recovery from geothermal brine in a manuscript that was submitted to Environmental Science & Te...
Jiao, Y. et al Lawrence Livermore National Laboratory
Jan 01, 2018
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Surface Complexation Modeling of Terbium Biosorption onto E. Coli Bacterial Surfaces with Lanthanide Binding Tags

Lanthanide binding tags (LBTs) have been engineered onto native Escherichia coli (E. coli) bacterial surfaces to enhance extraction and recovery of rare earth elements (REEs). Three strains of E. coli were studied: (1) the native E. coli surface, (2) a mutant E. coli surface with ...
Jiao, Y. Lawrence Livermore National Laboratory
Apr 01, 2018
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Rare Earth Desorption Test with Citrate and Bicarbonate

LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 u...
Jiao, Y. et al Lawrence Livermore National Laboratory
Jun 01, 2017
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EGS Collab Experiment 1: Circulation Testing Processed data

This submission includes processed and reduced data for circulation testing that was conducted at the 164' fracture on the 4850 ft level of the Sanford Underground Research Facility. The circulation tests were done to test the flow through the 164' fracture in the EGS Collab Exper...
Fu, P. et al Lawrence Livermore National Laboratory
Apr 01, 2021
7 Resources
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Fully Coupled Geomechanics and Discrete Flow Network Modeling of Hydraulic Fracturing for Geothermal Applications

The primary objective of our current research is to develop a computational test bed for evaluating borehole techniques to enhance fluid flow and heat transfer in enhanced geothermal systems (EGS). Simulating processes resulting in hydraulic fracturing and/or the remobilization of...
Fu, P. et al Lawrence Livermore National Laboratory
Jan 01, 2011
2 Resources
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Utah FORGE 2-2446: Closing the Loop Between In-situ Stress Complexity and EGS Fracture Complexity Workshop Presentation

This is a presentation on the Closing the Loop Between In-situ Stress Complexity and EGS Fracture Complexity project by Lawrence Livermore National Laboratory, presented by Dr. Matteo Cusini. The project's objective was to employ a combination of high-fidelity simulations and true...
Cusini, M. and Bunger, A. Lawrence Livermore National Laboratory
Sep 08, 2023
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Utah FORGE 5-2428: Fracture Permeability Impact on Reservoir Stress and Seismic Slip Behavior Workshop Presentation

This is a presentation on the Fracture Permeability Impact on Seismic Slip Behavior project by Lawrence Livermore National Laboratory, presented by Dr. Kayla A. Kroll. The project's objective is to develop, apply and validate a holistic thermal, hydrologic, mechanical, and chemic...
Kroll, K. et al Lawrence Livermore National Laboratory
Sep 08, 2023
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