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Rare Earth Desorption Test with Citrate and Bicarbonate
LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 u...
Jiao, Y. et al Lawrence Livermore National Laboratory
Jun 01, 2017
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Rare Earth Element Biomining from the Great Salt Lake Brine Using Engineered E. Coli
This data describes rare earth element adsorption onto E. coli cells engineered to express a lanthanide binding tag (LBT). We used a Great Salt Lake synthetic solution as the background matrix with Tb added to 1-10,000 ppb, concentrations much lower than the competing ions presen...
Jiao, Y. et al Lawrence Livermore National Laboratory
Jun 04, 2017
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Rare Earth Adsorption and Desorption with PEGDA Beads
We synthesized PEGDA polymer hydrogel beads for cell embedding and compared REE biosorption with these beads via a gravity-driven flow through setup. One way to set up a flow through system is by cell encapsulation into polymer beads with a column setup similar to that used in th...
Jiao, Y. et al Lawrence Livermore National Laboratory
Mar 01, 2017
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S-Layer Nanosheet Binding of Zn and Gd
This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+ or Gd3+-binding domains on these sheets. Their ab...
Ajo-Franklin, C. et al Lawrence Berkeley National Laboratory
Apr 15, 2016
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Chelating Resins for Selective Separation and Recovery of Rare Earth Elements from Low Temperature Geothermal Water
Study on the use of organic ligands to extract lanthanides from low temperature geothermal water.
Callura, J. Carnegie Mellon University
Dec 31, 2016
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